p-ISSN: 1300-0551
e-ISSN: 2587-1498

Halil İbrahim Kaya1, Cem Çetin2, Duygu Doğuç Kumbul3, Mustafa Onur Serbest4, Ali Erdoğan5

1Sports Medicine Section, Samsun Training and Research Hospital, University of Health Sciences, Samsun, Turkey
2Department of Sports Medicine, Faculty of Medicine, Süleyman Demirel University, Isparta, Turkey
3Department of Medical Biochemistry, Faculty of Medicine, Süleyman Demirel University, Isparta, Turkey
4Sports Medicine Section, Aydın State Hospital, Aydın, Turkey
5Sports Medicine Unit, Gloria Sports Arena, Antalya, Turkey

Keywords: Lactate analysis, validity, reliability


Objectives: The purpose of this study was to evaluate the reliabilities of the Biosen C Line (BCL) laboratory and Lactate Scout (LS) hand-held portable lactate analyser within a wide range lactate concentration. LS analyser was compared to the BCL analyser (reference method) to evaluate its validity.

Materials and methods: Blood samples were taken during an incremental treadmill test under laboratory conditions. In order to evaluate reliability, 99 blood samples with lactate concentrations changed between 0.8-11.1 mM were measured twice.

Results: For the LS analyser, intraclass correlation coefficient (ICC) was 0.994; typical error of measurement (TE) and coefficient of variation (CV) were 0.200 mM and 2.95%, respectively. For the BCL analyser, ICC was 1.000; TE and CV were 0.044 mM and 0.50%, respectively. LS hand-held portable- and BCL laboratory analysers’ reliability results show perfect accordance. In order to evaluate LS analyser reliability, 99 blood samples with lactate concentrations between 0.8-11.1 mM were also measured, giving a high validity coefficient of 0.976. Validity was also separately assessed for the three sample ranges of <2.5 mM, 2.5-5.0 mM and >5.0 mM, yielding ICC values of 0.862, 0.903 and 0.783, respectively. Comparing with the Bland & Altman method, LS and reference method mean lactic acid concentration difference was -0.097 mM, and limits of agreement -1.342 and 1.147 mM.

Conclusion: The results of the study revealed that the LS analyser has high validity, however at concentrations higher than 5.0 mM, its measures are found significantly lower than those of the reference method.

Cite this article as: Kaya HI, Cetin C, Doguc Kumbul D, Serbest MO, Erdogan A. Validity and reliability study of two different portable electro–enzymatic lactate analysers. Turk J Sports Med. 2020;55(2):138-47.

Financial Disclosure

Bu çalışma Süleyman Demirel Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından 3197-TU1-12 proje no ile desteklenmiştir.